HepG2 are adherent, epithelial-like cells growing as monolayers and in small aggregates, have a model chromosome number of 55. HepG2 cell line was derived from the liver tissue of fifteen year old male with differentiated hepatocellular carcinoma. Not tumorigenic in immunocompromised mice. Secrete plasma proteins, such as albumin, transferrin, fibrinogen, a-2-macroglobulin, plasminogen. Cells respond to stimulation with HGH.
Cytogenetics: human hyperdiploid karyotype - 52(47-54)<2n>XY, +2, +14, +17, +20, +2mar, t(1;21) (p22.2;p11-12), i(17q)/der(17)t(17;17)(p11;q11)
Copyright picture from altogen.com. Reproduced with permission from Altogen Biosystems.
1. Use Eagle's Minimum Essential Medium (DMEM and RPMI1640 also works well) supplemented with 10% FBS. Discard culture medium every 2-3 days
2. To passage cells, briefly rinse cell monolayer with 1xPBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution for 5 - 7 minutes
3. Once cell layer is dispersed (5-7 min at 37°C) deactivate Trypsin by adding equal volume of complete growth medium with 10% FBS
4. To avoid clumping don't agitate the cells by shaking the flask while waiting for the cells to detach
5. Split cells 1:4 every 3 days (recommended), or 1:8 every 6 days.
6. Cultures should be incubated at 37°C in humidified atmoshere with 5% CO2
Stable Cell Line Generation:
HepG2 cells have been demonstrated to be Neomycin G418 resistant (400 µg/mL).
siRNA Delivery - In Vivo Transfection Kits
CRO Pre-clinical Research Services: Xenograft animal models
GLP-compliant Cell Banking services
Generation of Stably Expressing Cell Lines in 28 Days
Stable RNAi Cell Line Generation: Stable Gene Knockdown
In Vivo siRNA Delivery: Tissue-targeted siRNA
Encapsulation of Protein, RNA, mRNA, and DNA Molecules into Liposomes
HepG2 cells: Research methods & Laboratory techniques: Link